Fig. 4: 2A binds directly to eukaryotic and prokaryotic ribosomes.
From: Structural and molecular basis for Cardiovirus 2A protein as a viral gene expression switch
a MST binding curves and apparent KD values using unlabelled 40S subunits at a concentration range of 20 pM–0.4 μM. All measurements were repeated as two independent experiments and error bars represent the standard deviation from the mean. 2A binds with high affinity to the small ribosomal subunit. b As in a with 60S subunits. Error bars as above. c Binding curve and apparent KD values using unlabelled 30S subunits at a concentration range of 30 pM–1 μM. Error bars as above. 2A shows a strong interaction with the prokaryotic small subunit. d As in c with 50S subunits at a concentration range of 27 pM–0.9 μM. e Binding curves and reported KD values for 2A-70S IC interactions. Error bars as above. f Same as e, with 2A and vacant 70S. Source data are provided as a Source Data file.
