Fig. 1: GPR180 is upregulated in BAT and required for the brown phenotype. | Nature Communications

Fig. 1: GPR180 is upregulated in BAT and required for the brown phenotype.

From: GPR180 is a component of TGFβ signalling that promotes thermogenic adipocyte function and mediates the metabolic effects of the adipocyte-secreted factor CTHRC1

Fig. 1: GPR180 is upregulated in BAT and required for the brown phenotype.

a Workflow of target identification by transcriptomic analysis of human supraclavicular BAT, subcutaneous WAT and hMADS cells differentiated into beige and white adipocytes. DESeq2 detects DE genes based on a generalized linear model using the negative binomial distribution. Effect of GPR180 silencing in human beige adipocytes on b UCP1 protein (n = 9; p < 0.0001) and c mitochondrial respiration (n = 5; p = 0.0016 for cAMP-stimulated uncoupled respiration). d Quantification of lentiviral GPR180 overexpression on mRNA level in white adipocytes (n = 3; p < 0.0001) and its effect on e UCP1 protein (n = 9; p = 0.0021) and f mitochondrial oxygen consumption (n = 5; p = 0.0437 for cAMP-stimulated uncoupled respiration and p = 0.0161 for maximal respiration). Data are presented as mean ± SEM. Statistical analysis was performed by two-sided Student´s t-test (b), one-way ANOVA with Dunnett’s post-hoc test (d, e) or two-way ANOVA with Tukey’s post-hoc test (c, f). Significance is indicated as *p < 0.05, **p < 0.01 and ***p < 0.001. cAMP cyclic adenosine monophosphate, GPR180 G protein-coupled receptor 180, hMADS human multipotent adipose-derived stem cells, OCR oxygen consumption rate, RFP red fluorescent protein, scBAT supraclavicular brown adipose tissue, UCP1 Uncoupling protein 1.

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