Fig. 2: Hepatic deletion of Mettl3 accelerates diet-induced NASH.

a METTL3 protein levels in livers of Mettl3^flox/flox and Mettl3-HKO mice at 8 weeks old (n = 3 for each group; P = 0.0059). The samples were derived from the same experiment and the blots were processed in parallel. b Liver weights of Mettl3^flox/flox and Mettl3-HKO mice at 11 weeks old (n = 9 for each group). c Liver TAG levels in Mettl3^flox/flox and Mettl3-HKO mice at 11 weeks old (Mettl3^flox/flox, n = 9; Mettl3-HKO, n = 8). d Serum ALT activity in Mettl3^flox/flox and Mettl3-HKO mice fed an HFD for 12 weeks (Mettl3^flox/flox, n = 14; Mettl3-HKO, n = 13; P = 0.0332). e Liver weights of Mettl3^flox/flox and Mettl3-HKO mice fed an HFD for 12 weeks (Mettl3^flox/flox, n = 8; Mettl3-HKO, n = 10; P = 0.0135). f Representative pictures and H&E staining of livers from Mettl3^flox/flox and Mettl3-HKO mice fed an HFD for 12 weeks. g Liver TAG levels in Mettl3^flox/flox and Mettl3-HKO mice fed an HFD for 12 weeks (Mettl3^flox/flox, n = 9; Mettl3-HKO, n = 11; P = 0.0007). h Serum ALT activity in Mettl3^flox/flox and Mettl3-HKO mice fed an MCD for 3 weeks (Mettl3^flox/flox, n = 9; Mettl3-HKO, n = 10; P = 0.0046). i Liver weights of Mettl3^flox/flox and Mettl3-HKO mice fed an MCD for 3 weeks (Mettl3^flox/flox, n = 8; Mettl3-HKO, n = 7; P = 0.0127). j Representative pictures and H&E staining of livers from Mettl3^flox/flox and Mettl3-HKO mice fed an MCD for 3 weeks. k Liver TAG levels in Mettl3^flox/flox and Mettl3-HKO mice fed an MCD for 3 weeks (Mettl3^flox/flox, n = 9; Mettl3-HKO, n = 10; P = 0.0006). l Sirius Red staining of liver sections from Mettl3^flox/flox and Mettl3-HKO mice fed an HFD for 12 weeks (n = 6 for each group; P = 0.0002). m F4/80 immunostaining of liver sections from Mettl3^flox/flox and Mettl3-HKO mice fed an HFD for 12 weeks (n = 4 for each group; P = 0.0439). n RT-qPCR analysis of mRNA levels in the livers of Mettl3^flox/flox and Mettl3-HKO mice fed an HFD for 12 weeks (Mettl3^flox/flox, n = 10–15; Mettl3-HKO, n = 12–14; Collagen1A1, P = 0.0061; αSMA, P = 0.0163; Mmp9, P = 0.0884; Tgfb1, P = 0.0001). o Sirius Red staining of liver sections from Mettl3^flox/flox and Mettl3-HKO mice fed an MCD for 3 weeks (Mettl3^flox/flox, n = 5; Mettl3-HKO, n = 6; P = 0.00004). p F4/80 immunostaining of liver sections from Mettl3^flox/flox and Mettl3-HKO mice fed an MCD for 3 weeks (n = 5 for each group; P = 0.0446). q RT-qPCR analysis of mRNA levels in the livers of Mettl3^flox/flox and Mettl3-HKO mice fed an MCD for 3 weeks (Mettl3^flox/flox, n = 10–11; Mettl3-HKO, n = 11–12; Collagen1A1, P = 0.0004; αSMA, P = 0.0015; Mmp9, P = 0.0062; Tgfb1, P = 0.0151). n was the number of biologically independent mice. Data represent the mean ± SEM. Significance was determined by unpaired two-tailed Student’s t test analysis. *P < 0.05. **P < 0.01. Source data are provided as a Source Data file.