Fig. 3: Hepatic deletion of Mettl3 enhances CD36-mediated free fatty-acid uptake.

a Relative mRNA levels in livers of Mettl3^flox/flox and Mettl3-HKO mice at 8 weeks old were determined by RT-qPCR (Mettl3^flox/flox, n = 12–13; Mettl3-HKO, n = 11; Fatp2, P = 0.0171; Fatp5, P = 0.0029; Cd36, P < 0.00001; Cpt1α, P = 0.4018; Mcad, P = 0.0025; Pparα, P = 0.2124; Fasn, P = 0.0393; Scd1, P = 0.5119; Srebp1, P = 0.02599; Chrebp, P = 0.00298; Pparg, P = 0.6043; mtGPAT1, P = 0.358; Dgat1, P = 0.1546; ApoB, P = 0.0453; Mttp, P = 0.0594). b CD36 protein levels in Mettl3^flox/flox and Mettl3-HKO mice at 8 weeks age were measured by immunoblotting. CD36 protein levels were quantified by ImageJ and normalized to Tubulin (n = 3 for each group; P = 0.0014). The samples were derived from the same experiment and the blots were processed in parallel. c Representative BODIPY FL C16 fluorescence image and relative lipid uptake levels in Mettl3^flox/flox and Mettl3-HKO mice at 8 weeks old (Mettl3^flox/flox, n = 7; Mettl3-HKO, n = 6; P = 0.02). d Representative BODIPY FL C16 fluorescence image and relative lipid uptake levels in primary hepatocytes isolated from Mettl3^flox/flox and Mettl3-HKO mice at 8 weeks old (n = 5 for each group; P = 0.00001). n was the number of biologically independent mice or cell samples. The cell culture experiments were repeated for three times independently with similar results. Data represent the mean ± SEM. Significance was determined by unpaired two-tailed Student’s t test analysis. *P < 0.05. **P < 0.01. Source data are provided as a Source Data file.