Fig. 4: Liver-specific Knockout of Mettl3 accelerates diet-induced liver injury.
From: The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression
a The TUNEL-positive cells in Mettl3flox/flox and Mettl3-HKO mice fed an HFD for 12 weeks (n = 5 for each group; P = 0.0474). b The TUNEL-positive cells in Mettl3flox/flox and Mettl3-HKO mice fed an MCD for 3 weeks (n = 5 for each group; P = 0.0027). c Cleaved caspase3 levels were measured by immunoblotting in the livers of Mettl3flox/flox and Mettl3-HKO mice fed either an HFD or MCD. Cleaved caspase3 levels were quantified by ImageJ and normalized to Tubulin (n = 3 for each group; HFD, Mettl3flox/flox versus Mettl3-HKO, P = 0.0197; MCD, Mettl3flox/flox versus Mettl3-HKO, P = 0.0013). The samples were derived from the same experiment and the blots were processed in parallel. d Caspase3 activity in the livers of Mettl3flox/flox and Mettl3-HKO mice fed either an HFD or MCD was measured by using a Caspase3 Assay Kit (ab39383, Abcam) following the instruction (Mettl3flox/flox, n = 6 for HFD, n = 5 for MCD; Mettl3-HKO, n = 5; HFD, Mettl3flox/flox versus Mettl3-HKO, P = 0.0034; MCD, Mettl3flox/flox versus Mettl3-HKO, P = 0.0433). n was the number of biologically independent mice. Data represent the mean ± SEM. Significance was determined by unpaired two-tailed Student’s t test analysis. *P < 0.05. **P < 0.01. Source data are provided as a Source Data file.
