Fig. 2: Systemic deletion of Col1 by CMV-Cre transgene results in early embryonic lethality. | Nature Communications

Fig. 2: Systemic deletion of Col1 by CMV-Cre transgene results in early embryonic lethality.

From: Type-I collagen produced by distinct fibroblast lineages reveals specific function during embryogenesis and Osteogenesis Imperfecta

Fig. 2

a Genetic strategy to achieve systemic (whole-body) knockout of type I collagen α1 chain (Col1a1) by crossing the Col1a1loxP/loxP mice with generic CMV-Cre mice. b Genotype distribution in live offspring examined at the time of weaning from the crosses between CMV-Cre;Col1a1loxP/+ and Col1a1loxP/loxP mice. c, d Hematoxylin and eosin (H&E) staining of the embryos of CMV-Cre-negative;Col1a1loxP/loxP (WT; wild-type) and CMV-Cre;Col1a1loxP/loxP (Col1a1cmvKO) at embryonic day E9.5 (c) and E12.5 (d). Col1a1cmvKO embryo of E9.5 was indicated by dashed line, with labeled epiblast and trophoblast areas. Representative images were shown for WT embryos (n = 6) and Col1a1cmvKO embryos (n = 6) of E9.5 (c); and WT embryos (n = 6) and Col1a1cmvKO embryos (n = 3, limited number of obtained embryos due to lethality) of E12.5 (d). e Genotype distribution in live offspring examined at E9.5 and E12.5 from the crosses between CMV-Cre;Col1a1loxP/+ and Col1a1loxP/loxP mice. Scale bars of whole-mount sections, 1 mm; Scale bars at 50× magnification, 500 μm; Scale bars at 200× magnification, 100 μm; Scale bars at 630× magnification, 50 μm.

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