Fig. 5: Specific deletion of Col1 in Fsp1-lineage cells causes Osteogenesis Imperfecta phenotype. | Nature Communications

Fig. 5: Specific deletion of Col1 in Fsp1-lineage cells causes Osteogenesis Imperfecta phenotype.

From: Type-I collagen produced by distinct fibroblast lineages reveals specific function during embryogenesis and Osteogenesis Imperfecta

Fig. 5

a Genetic strategy to delete Col1a1 specifically in Fsp1-lineage cells by crossing the Col1a1loxP/loxP mice with Fsp1-Cre mice. b Survival of wild-type (WT; with genotype of Fsp1-Cre-negative;Col1a1loxP/loxP) and Col1a1fspKO (Fsp1-Cre;Col1a1loxP/loxP) mice (n = 24 and 26, respectively). Kaplan-Meier plots and the log rank Mantel-Cox test were used to evaluate statistical differences of survival. ****P < 0.0001. c Micro-CT scanning of 6-month-old WT and Col1a1fspKO mice (with white arrows indicating the fractures in pelvis and ischium of Col1a1fspKO mice). d Radiograph of Col1a1fspKO mice at the age from 1 to 6 month. Radiograph of age-matched WT littermates was shown in Supplementary Fig. 7a. e Summary of the occurrence of fractures in pelvis, ischium, and/or joints, as examined by radiograph, of WT (n = 6) and Col1a1fspKO (n = 8) mice at the age from 1 to 6 month. f Body weight of WT and Col1a1fspKO mice (n = 6 mice per group) at the age of 3, 12, and 24 month. The unpaired, two-tailed t test was used to compare the mean of two independent groups. ***P = 9.57E-05 (female 12 weeks), 7.15E-07 (female 24 weeks), 0.00096 (male 12 weeks), 2.35E-07 (male 24 weeks). Data are represented as mean ± SEM. g The micro-CT images of trabecular and cortical bones of femurs from 6-month-old WT mice (n = 4) and Col1a1fspKO mice (n = 3). The trabecular bone material property measurements are compared as bone mineral density (BMD; ***P = 1.04E-05), bone volume/total volume (BV/TV; * P = 0.0289), bone surface area/bone volume (BS/BV; **P = 0.00212), trabecular thickness (Tb.Th; *P = 0.0287), and trabecular number (Tb.N; *P = 0.0160). The unpaired, two-tailed t test was used to compare the mean of two independent groups. Data are represented as mean ± SEM. The cortical bone material property measurements are continued in Supplementary Fig. 7d. h Biomechanical (three-point bending) test results of femurs from 6-month-old WT mice (n = 7) and Col1a1fspKO mice (n = 5) were presented as ultimate load (**P = 0.00135), yield load (***P = 0.00012), post-yield energy (***P = 2.01E-05), and post-yield displacement (***P = 0.00077). The unpaired, two-tailed t test was used to compare the mean of two independent groups. Data are represented as mean ± SEM.

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