Fig. 6: Histological analysis of bone tissues from Col1a1fspKO mice and Fsp1-Cre;LSL-YFP mice.
a, b H&E staining and Safranin-O/Fast Green staining of the forelimb elbow joints of 6-month-old WT and Col1a1fspKO mice (a). Quantification of % positive area for Safranin-O/Fast Green staining was based on three mice per group (b). The unpaired, two-tailed t test was used to compare the mean of two independent groups. **P = 0.00946. Data are represented as mean ± SEM. Scale bars at 50× magnification, 500 μm; Scale bars at 200× magnification, 100 μm. c Picrosirius Red staining of collagen content in the forelimb elbow joints from six-month-old WT and Col1a1fspKO mice (n = 3 mice per group) examined by circularly polarized light microscopy. Scale bars, 100 μm. d Representative immunofluorescence images of humerus serial sections from lineage-tracing Fsp1-Cre;LSL-YFP mice (n = 3 mice at two-month-old) stained for Fsp1-Cre-induced YFP. Scale bars, 50 μm. e Representative H&E and immunofluorescence images of humerus serial sections from Fsp1-Cre;LSL-YFP mice (n = 3 mice at two-month-old) stained for osteoblast marker osteocalcin (red) and Fsp1-Cre-induced YFP (green). Scale bars, 50 μm.
