Fig. 7: Single-cell RNA-sequencing analysis of bone marrow fractions from Col1a1fspKO mice.
a–c Single-cell RNA-sequencing analysis of cell mixture of bone marrow fractions from 2-month-old WT and Col1a1fspKO mice (n = 2 per group). Functional clusters of cells were defined with group definition listed, as implemented in the Seurat R package (a). Mono/Macro: monocyte/macrophage cluster. HSPCs: hematopoietic stem and progenitor cells. Dendritic: dendritic cell cluster. Eosino/Baso: eosinophil/basophil cluster. b Pie charts showing the percentage of each of the functional cell clusters in bone marrow fractions from WT and Col1a1fspKO mice. Total neutrophil clusters containing 3 subpopulations (1–3) were further plotted as pie charts. (c) List of top signature genes of “Neutrophil-1” cluster shown in (a) and (b), presented with P value (based on the non-parameteric Wilcoxon Rank Sum test) and Log2 fold change. Continued in Supplementary Fig. 8a–c. d Expression profile of Mmp9, Cxcl12, and Retnlg among defined cell clusters in the bone marrow fractions from WT and Col1a1fspKO mice shown in UMAP plot. Continued in Supplementary Fig. 8d. e, f Top upregulated genes of Neutrophil-1 cluster in Col1a1fspKO mice than WT mice shown in heat map plot (e) or violin plot (f). g Representative immunofluorescence images and quantification results of humerus bone marrow from two-month-old WT and Col1a1fspKO mice (n = 5 per group) stained for Neutrophil-1 marker Ly6G (yellow). Scale bars, 50 μm. The unpaired, two-tailed t test was used to compare the mean of two independent groups. ***P = 0.00096. Data are represented as mean ± SEM.
