Fig. 3: DLG2−/− lines display deficits in neuron morphology & migration.
a The number of primary neurites (projecting from the soma). Neither genotype (F1,126 = 1.591; P = 0.2095) nor time (F1,126 = 2.278; P = 0.1337) had significant effects the numbers of primary neurites. b The number of secondary neurites (projecting from primary neurites). Genotype (F1,126 = 18.78, P = 2.97 × 10−5) had a significant effect on number of secondary neurites, while time (F1,126 = 1.082, P = 0.3003) did not. c The total neurite length. Both genotype (F1,126 = 4.568; P = 0.0345) and time (F1,126 = 26.33; P = 1.06 × 10−6) had significant effects on total neurite length. However, post hoc analysis showed no significant differences at individual timepoints. d The soma area. Neither genotype (F1,136 = ; P = 0.9170) nor time (F1,136 = 1.399; P = 0.2390) had a significant effect on soma area. For a–d WT n = 32, 38 cells from 3 independent experiments, days 30 & 70 respectively; KO n = 32, 28 cells from 3 independent experiments, days 30 & 70 respectively. e Representative traces showing the neuronal morphology. f The average speed of neuronal migration over 70 h, from day 40 of cortical differentiation. DLG2−/− neurons showed significantly decreased average migration speed compared to WT (t52 = 6.1175; P = 1.26 × 10−7; n = 27). For f–g both WT & KO n = 27 cells from 3 independent experiments. g The displacement of neurons at 70 h migration. DLG2−/− neurons showed significantly decreased displacement compared to WT (t52 = 3.244; P = 0.0021; n = 27). h Representative traces of neuronal migration from a given origin over 70 h. Morphology data sets (a–d) were analyzed by two-way ANOVA with post hoc comparisons using Bonferroni correction, comparing to WT controls. Migration data sets (f, g) were analyzed by unpaired two-tailed Student’s t test. Stars above bars represent, **P < 0.01; ****P < 0.0001 vs. WT control (Bonferroni-corrected for morphology analyses). Source data are provided as a Source Data file.
