Fig. 1: PP2A loss or inhibition enhances cytotoxic T cell infiltration in colorectal cancer. | Nature Communications

Fig. 1: PP2A loss or inhibition enhances cytotoxic T cell infiltration in colorectal cancer.

From: Protein phosphatase 2A inactivation induces microsatellite instability, neoantigen production and immune response

Fig. 1

a, b Ppp2r1a-loss colon tumours were induced in Lgr5-EGFP-CreERT2; Ppp2r1aflox/flox by treatment with DMBA and tamoxifen for 36 days. Control colon tissues (Control) and Ppp2r1a-loss colon tumours were harvested for analysis. a Representative images of immunofluorescence showing increased CD8+ and CD20+ and decreased Foxp3+ infiltration in murine Ppp2r1a-loss colon tumours compared to controls. Arrows indicate positive signals. Images are representative of three biological independent samples for each group. Bar = 25 μm. b Gene set enrichment analysis (GSEA) for cytokine, chemokine, IFN-gamma response, and JAK-STAT signalling pathways in murine Ppp2r1a-loss colon tumours compared to controls. c TCGA-COAD (n = 461) and d TCGA-READ (n = 172) omics analysis revealed that CIP2A and SET levels were significantly positively correlated with CD8+, CD4+, and B cell infiltration levels and significantly negatively correlated with Treg infiltration, while PPP2R1A levels were negatively correlated with CD8+ infiltration but positively correlated with Treg infiltration. Results are determined by the Spearman correlation. Source data are provided as a Source data file.

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