Fig. 4: Identification of a novel NUFIP body.
From: An assembly of nuclear bodies associates with the active VSG expression site in African trypanosomes
a Schematic of NUFIP body components: NUFIP, RBP34, ZNHIT3 and the newly identified NufB1 and NufB2 proteins. Relevant protein domains and motifs were identified using PFAM, and disordered domains with PONDR (coloured boxes). b SR-SM imaging of a BF T. brucei cell line with three NUFIP components epitope-tagged. Signals in the nuclei (dashed lines) correspond to NUFIP::mNG (NUFIP-mNG, green), mCh::NufB1 (NufB1-mCH, red) and NufB2::6HA (NufB2-HA, magenta), with DNA stained with DAPI (blue). The insets show further magnification of the foci, illustrating the ‘shell’-like structure. Scale bar = 1 μm for main images, and 200 nm for the insets. c As in (b), only showing SR-SM microscopy imaging of a BF cell line with two NUFIP body components epitope-tagged: NufB2::6HA (NufB2-HA, magenta) and RBP34::mNG (RBP34-mNG, green). d As in (b), but showing SR-SM microscopy imaging of a BF cell line with two NUFIP body components epitope-tagged: NufB2::6HA (NufB2-HA, magenta) and ZNHIT3::mNG (ZNHIT3-mNG, green).
