Fig. 1: TCPTP is comprised of a structured N-terminal catalytic domain and an intrinsically disordered dynamic C-terminal domain. | Nature Communications

Fig. 1: TCPTP is comprised of a structured N-terminal catalytic domain and an intrinsically disordered dynamic C-terminal domain.

From: The catalytic activity of TCPTP is auto-regulated by its intrinsically disordered tail and activated by Integrin alpha-1

Fig. 1

a TCPTP construct. b Overlay of the 2D [1H,15N] TROSY spectra of (2H,15N)-labeled TCPTP (black) and TCPTPCAT (red). TCPTP IDR peaks (residues 303–387) are highlighted by dashed box. c Secondary-structure propensity (SSP) vs TCPTP residue in TCPTP and the isolated TCPTPTail. SSP reports transient secondary structure elements (SSP > 0, α helix; SSP < 0, β strand). d TCPTP structural models (right) generated by MultiFoXS32 based on SAXS data (left). SAXS scattering data (blue) is best represented by a 3-model ensemble (light pink). Inset bar graph highlighting that a 3-member ensemble is statistically necessary for the best fit (X2). The error bar indicates the range of X2 values for top 100 multistate models32. The computed output value along with error from MultiFoXS is provided in the Source Data file as Source data. The three ensemble members are shown in green; populations for the optimal fit are shown.

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