Fig. 2: A high-fat diet induces inflammation in eWAT.
From: Macrophages in epididymal adipose tissue secrete osteopontin to regulate bone homeostasis
a Representative H&E staining (left) and quantification of CLS area percentage (right, n = 4) of eWAT from NFD- and HFD-fed mice at the indicated time points. Scale bar: 100 μm. b Representative H&E staining (left) and quantification of CLS area percentage (right, n = 4) of iWAT from NFD- or HFD-fed mice at the indicated time points. Scale bar, 100 μm. c–e Relative expression of Tnfa, Il-1b, and Il-10 in eWAT (n = 4) (c), iWAT (n = 4) (d), and bone marrow (BM, n = 4) (e) over the course of the feeding regimen. n = 4 biologically independent samples per group. Images are representative of 3 independent experiments. All data are presented as mean ± SD. Two-way ANOVA with Sidak’s post-hoc test (a–e) were used. Source data are provided as a Source Data file.
