Fig. 3: Removal of bilateral eWAT (eWATx) alters bone mass and rBMAT mass.
From: Macrophages in epididymal adipose tissue secrete osteopontin to regulate bone homeostasis
a, b Representative μCT images (a) and quantification (n = 5 biologically independent samples) (b) of proximal tibiae from NFD- and HFD-fed mice that had undergone either sham surgery or removal of the bilateral eWAT (eWATx). Scale bar: 500 μm. c, d Representative μCT images (c) and quantification (n = 3 biologically independent samples) (d) of rBMAT in tibiae at week 8 of feeding and eWATx or not. Scale bar: 500 μm. e ITTs were performed at weeks 6, 8, and 12 of NFD- and HFD-fed mice that had undergone eWATx (n = 5 biologically independent samples). Images are representative of 3 independent experiments. All data are presented as mean ± SD. Two-way ANOVA with Tukey’s post-hoc test (b, d) and two-way ANOVA with Sidak’s post-hoc test (e) were used. Source data are provided as a Source Data file. See also Supplementary Tables 2 and 3.
