Fig. 4: Infiltrating ATMs in eWAT are related to bone marrow metabolism.

a Immunofluorescence staining of macrophages (F4/80) in eWAT after mice were injected with clodronate liposomes (CL) or control liposomes (Ctrl) into bilateral eWAT for 8 weeks. Green, F4/80; blue, DAPI stain for cell nuclei. Scale bar: 75 μm. b Quantification of the F4/80-positive area percentage with immunofluorescence staining from A (n = 5 biologically independent samples). c, d Representative μCT image (c) and quantification (n = 5 biologically independent samples) (d) of proximal tibiae after mice were injected with control liposomes or CL for 8 weeks. Scale bar: 500 μm. e μCT quantification of rBMAT in tibiae after mice were injected with control liposomes or CL for 8 weeks (n = 4 biologically independent samples). f Heatmap summarizing the fold changes in mRNA expression of different biomarkers in eWAT from NFD- and HFD-fed mice at week 12 (n = 4 biologically independent samples). g Relative expression of Spp1 in eWAT (n = 4 biologically independent samples) and iWAT (n = 4 biologically independent samples) over the course of feeding. Images are representative of 3 independent experiments. All data are presented as mean ± SD. Two-way ANOVA with Tukey’s post-hoc test (b, d, e) and two-way ANOVA with Sidak’s post-hoc test (g) were used. Source data are provided as a Source Data file. See also Supplementary Tables 4 and 5.