Fig. 4: CLSY3 and CLSY4 are required for 24nt-siRNA production and Pol-IV recruitment at siren loci.
From: The CLASSY family controls tissue-specific DNA methylation patterns in Arabidopsis
a Scaled Venn diagrams showing the relationships between siren 24nt-siRNA clusters and those with reduced expression in the indicated clsy mutants from ovule tissue. Siren loci are colored based on the 24nt-siRNA Class (Class 10) in which they reside based on Fig. 2c. b and c) Violin plots showing 24nt-siRNA or DNA methylation levels at siren loci (n = 133) in the indicated genotypes and tissues, respectively. Metaplots (upper) and heatmaps (lower) showing the levels of CLSY3 enrichment (d) or Pol-IV enrichment (e) at CLSY3 ChIP-seq peaks (n = 102) and surrounding genomic regions (± 2 kb). These heatmaps are all in the same order, based on a high to low ranking of CLSY3 enrichment in (c) as indicated by the inverted black triangle. For the Pol-IV ChIP data the IP subscripts indicate the genetic background (e.g. IPc3 indicates the clsy3 background). In d, the distribution of motif 1 is included on a second y-axis scale and the occurrences of this motif (n = 116), as well as the overlaps between the CLSY3 peaks and siren loci, clsy3-dependent, or clsy3,4-dependent 24nt-siRNA clusters are indicated to the right of the heatmap.
