Fig. 6: clsy mutants shift the epigenetic landscapes between tissues.
From: The CLASSY family controls tissue-specific DNA methylation patterns in Arabidopsis
a and c Principal Component Analysis (PCA) plot of 24nt-siRNA or DNA methylation levels in all contexts (all mC) at the 12,939 master 24nt-siRNA clusters compiled from all four tissues (Supplementary Data 5) or at the WTvsWT CHH DMRs (Supplementary Data 7) that overlap with these clusters, respectively. WT samples (three replicates; open circles) and various RdDM mutants (filled circles) are colored by tissue, as indicated at the bottom right or top left. Mutants are abbreviated as follows, c1 = clsy1 etc. b and d Heatmaps showing 24nt-siRNA and DNA methylation levels in all sequence contexts (all mC) at reduced 24nt-siRNA clusters grouped by the ten classes defined in Fig. 2c (b) or at the WTvsWT CHH DMRs within these clusters (d) for the genotypes and tissues indicated above. The color scales and the numbers (n) of clusters or DMRs are as indicated on the x- and y-axes, respectively. In all panels, the arrows show mutants with patterns that resemble other WT tissues (colored) or single mutants that mimic pol-iv (grey). In b and d, the classes that are upregulated or downregulated are indicated above the colored arrows.
