Fig. 1: Difference in the cell abundance and size of methanotrophic bacteria (Alpha-MOB and Gamma-MOB) and DAPI-stained prokaryotes across incubations with water from different depths in the six studied lakes.
From: The role of methanotrophy in the microbial carbon metabolism of temperate lakes
a Cell abundance across incubations (DAPI: n = 32, Alpha-MOB: n = 32, Gamma-MOB: n = 32). b Average cell size in each incubation (DAPI: n = 32, Alpha-MOB: n = 32, Gamma-MOB: n = 32). Boxplots show median, first and third quartiles (hinges), and 1.5 x interquartile range (whiskers). Different letters indicate significant difference between means in a (p < 0.001, ANOVA (F-value = 50.97) and p < 0.001, Tukey HSD) and in b (p < 0.001, ANOVA (F-value = 35.48) and p < 0.001, Tukey HSD). c, d Microscopic picture of Gamma-MOB cells visualized by CARD-FISH and corresponding picture of the same field of view showing all prokaryotic cells visualized by DAPI staining, respectively. e, f Microscopic picture of Alpha-MOB cells visualized by CARD-FISH and corresponding picture of the same field of view showing all prokaryotic cells visualized by DAPI staining, respectively. White scale bars in c to f represent 20 μm. Note log scale in y axis of a.
