Fig. 3: Optical activation of the PPC cell ensemble generates an artificial pre-exposed context-IS associative memory. | Nature Communications

Fig. 3: Optical activation of the PPC cell ensemble generates an artificial pre-exposed context-IS associative memory.

From: A cortical cell ensemble in the posterior parietal cortex controls past experience-dependent memory updating

Fig. 3

a Labeling of the PPC neurons in c-fos-tTA transgenic mice with the LVs TRE3G-ChR2-EYFP or TRE3G-EYFP. b Activating cell ensembles during an IS session of conditioning (top). The blue and gray bars indicate the presence or absence of Dox, respectively. The graph shows freezing level in context A or C in the conditioning and test session (bottom) (ChR2-Light OFF in circle, n = 17 mice, ChR2-Light ON in circle, n = 19 mice, EYFP-Light ON in circle, n = 9 mice, ChR2-Light OFF in triangle, n = 7 mice, ChR2-Light ON in triangle, n = 6 mice) (two-way ANOVA with RM followed by Sidak’s post hoc test, session: P < 0.0001, group: P < 0.0001, session × group: P < 0.0001). Mice were sacrificed 90 min after the test for immunohistochemistry (Fig. 6c, d). Error bars represent the mean ± s.e.m. *P < 0.05. Ctx context, IS immediate shock, IHC immunohistochemistry. For details of statistical data, see Supplementary Table 1. Source data are provided as a Source Data file.

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