Table 1 TCRs expressed by SGL-specific T Cell Lines.

From: CD4 and CD8 co-receptors modulate functional avidity of CD1b-restricted T cells

Lines

TCR

Variable

CDR3

Joining

A01

ɑ

TRAV8-6

CAVKAGYSSASKIIF

TRAJ3

 

β

TRBV12-4

CASKGKQGPEQFF

TRBJ2-1

A05

ɑ

TRAV13-2

CAEVLGGTSYGKLTF

TRAJ52

 

β

TRBV20-1

CSASGRRGYGYTF

TRBJ1-2

  1. Clonality was determined by staining with antibodies targeting human TCR-β variable genes (IOTest BetaMark Kit, Beckman Coulter) or high-throughput sequencing (ImmunoSEQ, Adaptive Biotechnologies). The full-length TCR sequence was determined by template-switched PCR after sorting tetramer-positive T cells. The T cell receptor gene usage and CDR3 region amino acid sequence is summarized here. Variable and joining gene segment names were assigned using the International ImMunoGeneTics (IMGT) Database.
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