Fig. 5: Application of iProChip-DIA for proteomic profiling of MEC-1 cells. | Nature Communications

Fig. 5: Application of iProChip-DIA for proteomic profiling of MEC-1 cells.

From: Streamlined single-cell proteomics by an integrated microfluidic chip and data-independent acquisition mass spectrometry

Fig. 5: Application of iProChip-DIA for proteomic profiling of MEC-1 cells.

a Identification summary of protein groups across different MEC-1 cell numbers by iProChip coupled to DIA-MS. Data are presented as mean values ± SD (n = 3 independent cell samples for each condition). b Assessment of dynamic range based on protein abundance rank and annotation of selected proteins related to immune cancer markers. c Enrichment of immune-related and other functional classes against the UniProtKB database. d Kinase tree for mapping 114 kinases from the total cell numbers. The kinase families listed includes TK (tyrosine kinases), TKL (tyrosine kinase-like), CK1 (casein kinase 1), CAMK (calcium/calmodulin-dependent protein kinase), AGC (containing PKA, PKG, PKC families), CMGC (containing CDKs, MAPK, GSK, CLK families), and STE (serine/threonine kinases many involved in MAPK kinases cascade). Source data are provided as a Source data file.

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