Fig. 6: Single-cell integrated proteomics chip (SciProChip) for 20-plex single-cell proteomic analysis. | Nature Communications

Fig. 6: Single-cell integrated proteomics chip (SciProChip) for 20-plex single-cell proteomic analysis.

From: Streamlined single-cell proteomics by an integrated microfluidic chip and data-independent acquisition mass spectrometry

Fig. 6: Single-cell integrated proteomics chip (SciProChip) for 20-plex single-cell proteomic analysis.

a A photograph of SciProChip for parallel processing of 20 single cells. b A schematic of the overall SciProChip layout. The control layer is shown in pink, while the flow layer is shown in black and blue. Note that SciProChip contains 20 operational units. c A bright-field image of a capturing chamber with a trapped cell. Scale bar: 120 μm. d The cell usage efficiency of SciProChip. Data are presented as mean values ± SD (n = 28 independent measurements). e Protein group identification of 10 single cells from 2 independent experimental batches. SC: single cell. f Overlaps of protein groups identified in replicate analyses and g distributions of total protein abundances in identified proteins of single cells shown in e. Each data was analyzed as an individual measurement. Centerlines show the mean; box limits indicate the 25th and 75th percentiles; whiskers, 1.5× interquartile range. h Pairwise Pearson’s correlation showing the reproducibility of protein abundances obtained across different single cells. i Correlation between the estimated cell sizes and protein groups identified of 20 single cells. Source data are provided as a Source data file.

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