Fig. 1: Metagenomic and microscopic characterization of the community in subsurface fluids discharged by Geyser Andernach.
From: Genetic diversity in terrestrial subsurface ecosystems impacted by geological degassing
A RpS3-based phylogenetic diversity of the organisms in the Geyser Andernach. Centroid rpS3 sequences (after clustering at 99% similarity using cdhit) were used for the calculation of the phylogenetic tree using IQTree. The colors of the different branches signify different phyla. Matching recovered draft genomes in each sample (A–C for samples GA_E1-1, GA_E1-2, and GA_E2-1, respectively), i.e., genomes binned from these samples, are provided as green boxes (otherwise left white). The presence of marker genes based on a marker gene search using HMMs on these genomes for specific chemolithoautotrophic pathways is shown as green boxes (otherwise left white). C signifies carbon fixation with (1) CBB, (2) rTCA, and (3) WL, C1 for C1-metabolism with (4) carbon monoxide oxidation, (5) formaldehyde oxidation, and (6) methanol oxidation, O for oxygen metabolism with (7) cytochrome c bd, (8) cytochrome c bo, (9) cytochrome c caa3, and (10) cytochrome cbb3, H for hydrogen metabolism with (12) FeFe-Hydrogenases type A, (13) NiFe-Hydrogenases type 3b, (14) NiFe-Hydrogenases type 3c, (15) Nife-Hydrogenases, (16) NiFe-Hydrogenases type 4 and (17) NiFe-Hydrogenases type 1, N for nitrogen metabolism with (18) Nitrate reduction, (19) Nitric oxide reduction, (20) nitrite reduction and (21) nitrous oxide reduction, S for sulfur metabolism with (22) sulfide oxidation, (23) sulfite reduction with dsr, (24) sulfite reduction with asr, (25) sulfur oxidation with dsr, (26) sulfur oxidation with sor, (27) sulfur oxidation with sdo, (28) sulfate reduction via APS with sat and (29) Thiosulfate disproportionation. Olive bars show the average iRep value of the respective bacterial population, brown bars show the maximal growth rate of the representative genome as estimated by growth red, and blue bars show the average log10-scaled coverage. B Morphologies of microorganisms as determined via DAPI staining and fluorescence microscopy (scale bars = 5 µm) are shown. The morphologies were documented in two sampling campaigns (June 2016 and February 2018 with three and two samples in technical duplicates, respectively).
