Fig. 1: C. elegans harbors three homologs of mammalian PGP/G3PP with glycerol-3-phosphate phosphatase function. | Nature Communications

Fig. 1: C. elegans harbors three homologs of mammalian PGP/G3PP with glycerol-3-phosphate phosphatase function.

From: Phosphoglycolate phosphatase homologs act as glycerol-3-phosphate phosphatase to control stress and healthspan in C. elegans

Fig. 1

a Sequence alignment of PGP/G3PP was performed using Clustal Omega. Regulatory motifs are indicated and the cap domain is highlighted. b Glycerol content in wild-type (WT) and pgph-1; pgph-2; pgph-3 synchronized L4/young adult animals grown on NGM plates. Data represent mean ± SEM, three biological replicates, and three independent experiments. c Violin plots of relative metabolite levels and ratios of some metabolites in WT and pgph-1; pgph-2; pgph-3 synchronized L4/young adult animals grown on NGM plates. Lines in violin plots denote the median of the analyzed groups and gray circles indicate individual data points (four biological replicates per group per independent experiment and four independent repeats). d, e Oil red O staining and quantification in WT and pgph-1; pgph-2; pgph-3 mutant 1-day adult animals. Data represent mean ± SEM from three independent experiments (WT, n = 83 and pgph-1; pgph-2; pgph-3, n = 81). Scale bars represent 50 µm. f Triglyceride levels in WT and pgph-1; pgph-2; pgph-3 1-day adult synchronized animals. Data represent mean ± SEM, three biological replicates per group per independent repeat, and three independent experiments. In all figures, ****P < 0.0001 and P-values were obtained by student’s two-tailed t-test. Data are provided as a Source Data file.

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