Fig. 2: Enhanced lipogenesis is required for the fat accumulation in triple pgph mutant animals. | Nature Communications

Fig. 2: Enhanced lipogenesis is required for the fat accumulation in triple pgph mutant animals.

From: Phosphoglycolate phosphatase homologs act as glycerol-3-phosphate phosphatase to control stress and healthspan in C. elegans

Fig. 2

a Scheme showing the lipogenesis pathway in C. elegans highlighting in yellow the genes that are significantly induced in pgph-1; pgph-2; pgph-3 mutant animals at basal level. b, c Oil red O staining and quantification in WT and pgph-1; pgph-2; pgph-3 mutant 1-day adult animals treated or not with fasn-1 RNAi (b) or fat-7 RNAi (c). Scale bars indicate 50 µm. Sample numbers for b: n = 76 (WT-Ev), n = 59 (pgph-1; pgph-2; pgph-3-Ev) and n = 93 (WT-fasn-1 RNAi), n = 50 (pgph-1; pgph-2; pgph-3 - fasn-1 RNAi). Sample numbers for c: n = 45 (WT-Ev), n = 42 (pgph-1; pgph-2; pgph-3-Ev), and n = 47 (WT-fat-7 RNAi), n = 39 (pgph-1; pgph-2; pgph-3-fat-7 RNAi). Data represent mean ± SEM from two independent experiments. P-values were obtained by one-way ANOVA with the Bonferroni test. d Relative expression of indicated genes in WT animals and pgph-1; pgph-2; pgph-3 synchronized young adult animals. Genes that are significantly induced are highlighted in yellow. Data represent mean ± SEM, n = 4 independent experiments. P-values were obtained by the student’s two-tailed t-test. Significance in all figures: ****P < 0.0001. Data are provided as a Source Data file.

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