Fig. 3: PGPH/G3PP is required for glycerol production to allow resistance to hyperosmotic stress. | Nature Communications

Fig. 3: PGPH/G3PP is required for glycerol production to allow resistance to hyperosmotic stress.

From: Phosphoglycolate phosphatase homologs act as glycerol-3-phosphate phosphatase to control stress and healthspan in C. elegans

Fig. 3

a Relative expression of indicated genes in WT animals treated or not with 400 mM NaCl for 3 h. Data represent mean ± SEM, four independent experiments. P-values are obtained by unpaired two-tailed student’s t-test. b Violin plots representing glycerol levels of WT and pgph-1; pgph-2; pgph-3 synchronized L4/young adults exposed or not to 400 mM NaCl for 3 h. In all groups, n = 9. Plots are generated with three biological replicates per group per independent repeat and a total of three independent experiments. Lines in the violin plots represent median of corresponding groups. P-values are obtained by one-way ANOVA with the Bonferroni test. c Relative metabolite levels and ratios of some metabolites in WT and pgph-1; pgph-2; pgph-3 synchronized L4/young adult animals grown on NGM plates and exposed or not to 400 mM NaCl for 3 h. Data represent mean ± SEM, n = 8, four biological replicates per group per independent repeat and two independent experiments. P-values are obtained by one-way ANOVA with the Bonferroni test. d Survival curves of indicated strains to 400 mM NaCl. P-values are obtained by two-sided Mantel–Cox comparisons. Number of separate experiments, animals, and detailed statistics are shown in Supplementary Data 1. Significance in all figures: ****P < 0.0001. Data are provided as a Source Data file.

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