Fig. 5: PGPH loss shortens lifespan and healthspan in normal and high glucose conditions.

a Lifespan survival curves of WT animals treated with Ev or pgph RNAi. b Lifespan survival curves of indicated worm strains on NGM plates. c–e Locomotion analysis of worm body bends/s (c), speed (d), and peristaltic speed (e) of indicated worm strains at days 3, 5, 7, and nine of age. Data is represented using violin box plots from three independent experiments with indicated median lines. More than 150 tracks are analyzed per condition and an exact number of tracks is shown in the data source. f, g Survival curves of indicated worm strains grown on plates supplemented with 2% glucose. h–j Locomotion analysis of worm body bends/s (h), speed (i), and peristaltic speed (j) of indicated worm strains grown on plates supplemented with 2% glucose at days 3, 5, 7, and 9 of age. Data is represented using violin box plots from three independent experiments with indicated median lines. More than 150 tracks were analyzed per condition and an exact number of tracks is shown in the data source. Number of separate lifespan and glucotoxicity experiments, animals, and detailed statistics are shown in Supplementary Data 2 and 3. Significance in all figures: ****P < 0.0001. P-values of lifespan and glucotoxicity experiments were obtained by two-sided Mantel–Cox. P-values of locomotion analysis were obtained by one-way ANOVA with the Bonferroni test. Data are provided as a Source Data file.