Fig. 6: pgph-2 overexpression improves healthspan and decreases fat content.

a Lifespan of pgph-2 overexpressing animals in comparison to control nematodes. Number of separate lifespan experiments, animals and detailed statistics are shown in Supplementary Data 2. P-value is obtained using the two-sided Mantel–Cox test. b–f Locomotion and body size analyses including the number of body bends per second (b), speed (c), peristaltic speed (d), mean length (e), and mean area (f) in pgph-2 overexpressing animals in comparison to control nematodes at days 3, 5, 7, and 9 of age. Data is shown using violin box plots with denoted median lines from three independent experiments. More than 150 tracks analyzed per condition and exact number of tracks is shown in data source. P-values are obtained by one-way ANOVA with Bonferroni test. g Violin plots showing pharyngeal pumping rates of control and pgph-2 overexpressing nematodes at days 2, 3, and 4 of age. Sample numbers: n = 35 (control day 2), n = 27 (pgph-2 o/e1 day 2), n = 37 (control day 3), n = 30 (pgph-2 o/e1 day 3), n = 36 (control day 4), n = 30 (pgph-2 o/e1 day 4). P-values are obtained by one-way ANOVA with Bonferroni test. h, i Oil red O staining (h) and quantification (i) in 3 days old adult control and pgph-2 overexpressing nematodes. Scale bars represent 50 µm. Data represent mean ± SEM from three independent experiments. Sample numbers: n = 66 (control), n = 44 (pgph-2 o/e1). P-value obtained with two-tailed student’s t-test. j, k Brood size (j) and egg laying (k) in pgph-2 overexpressing animals in comparison to control nematodes. P-value obtained with two-tailed student’s t-test. Data represent mean ± SEM n = 10, three independent experiments. Significance in all figures: ****P < 0.0001. Data are provided as a Source Data file.