Fig. 7: pgph-2 overexpression increases lifespan under excess glucose and reduces glucose-induced fat deposition.

a Survival curves of pgph-2 overexpressing animals in comparison to control nematodes in excess glucose conditions. Number of separate experiments, animals, and detailed statistics are shown in Supplementary Data 3. P-values are obtained by the two-sided Mantel–Cox test. b–f Locomotion and body size analyses including the number of body bends per second (b), speed (c), peristaltic speed (d), mean length (e), and mean area (f) in pgph-2 overexpressing animals in comparison to control nematodes grown on plates supplemented with 2% glucose at days 3, 5, 7, and 9 of age. Data is represented using violin box plots with denoted median lines from three independent experiments. More than 150 tracks were analyzed per condition and an exact number of tracks is shown in the data source. g Violin box plots showing pharyngeal pumping rates of control and pgph-2 overexpressing nematodes grown on plates supplemented with 2% glucose at the indicated age. Sample numbers: n = 36 (control day 2), n = 30 (pgph-2 o/e1 day 2), n = 34 (control days 3, 4, 7, 9, 12), n = 30 (pgph-2 o/e1 days 3, 4, 7, 9, 12). h Oil red O staining and quantification in 3 days old adult control and pgph-2 overexpressing nematodes grown on plates supplemented with 2% glucose. Scale bars represent 50 µm. Data represent mean ± SEM from three independent experiments. Sample numbers: n = 65 (control), n = 68 (control-2% glucose), n = 63 (pgph-2 o/e1). i, j Brood size (i) and egg laying (j) in indicated pgph-2 overexpressing animals grown on plates supplemented with 2% glucose in comparison to control nematodes. Data represent mean ± SEM, n = 10, three independent experiments. Significance in all figures: ****P < 0.0001. From b–j, P-values are obtained by one-way ANOVA with the Bonferroni test. Data are provided as a Source Data file.