Fig. 4: Re-expression of Scn1a gene after symptom onset rescues altered interactions with environment and social impairment in Dravet mice.

a Scheme of the open field arena (b) distance traveled (**p = 0.0041, *p = 0.0134, one way-ANOVA followed by Tukey’s multiple comparisons). c Velocity (**p = 0.0048, *p = 0.016, one way-ANOVA followed by Tukey’s multiple comparisons). d Percentage of time spent in home (two way-ANOVA). e Scheme of the arena with a novel object in the center. f Percentage of time spent in home, transition and exploration areas after that a novel object has been placed in the center of the arena; (*p < 0.05, **p = 0.006, ****p < 0.0001, two way-ANOVA followed by Tukey’s multiple comparisons). g Time spent sniffing the novel object (**p < 0.005, one way-ANOVA with Tukey’s). n = 19 for Scn1a^+/+, n = 20 for Scn1a^Stop/+-Ctrl and n = 15 for Scn1a^Stop/+ -Cre for open field test. h Scheme of the three chamber test. i Time spent in zone1 and zone 2 chambers when an unfamiliar mouse is placed in zone 1 chamber (****p < 0.0001, two-way ANOVA followed by Tukey’s multiple comparisons). j Sniffing. k Sociability index. (**p = 0.0010, one-way ANOVA followed by Tukey’s multiple comparisons). Data are shown as mean ± SEM, with dots representing individual mice, n = 18 for Scn1a^+/+, n = 18 for Scn1a^Stop/+-Ctrl and n = 15 for Scn1a^Stop/+ -Cre for three-chamber test. Source data are provided as a Source Data file. a, e, h, j were Created with BioRender.com.