Fig. 2: Applying the biosensor in its isobutanol configuration for high-throughput screening of a library of strains combinatorially transformed with the mitochondrial isobutanol biosynthetic pathway. | Nature Communications

Fig. 2: Applying the biosensor in its isobutanol configuration for high-throughput screening of a library of strains combinatorially transformed with the mitochondrial isobutanol biosynthetic pathway.

From: Biosensor for branched-chain amino acid metabolism in yeast and applications in isobutanol and isopentanol production

Fig. 2: Applying the biosensor in its isobutanol configuration for high-throughput screening of a library of strains combinatorially transformed with the mitochondrial isobutanol biosynthetic pathway.

a Isobutanol titers of 24 colonies randomly selected from unsorted (blue), first round sorted (red), or second round sorted (green) populations derived from a library of strains transformed with constructs overexpressing different combinations of the mitochondrial isobutanol pathway. All data are shown as mean values. Error bars represent the standard deviation of the titers of the 24 colonies analyzed. A two-sided t-test was used to determine the statistical significance of the differences in isobutanol titers of analyzed strains from each population; From left to right: P < 0.0001, P = 0.001; ***P ≤ 0.001, ****P ≤ 0.0001. b Histogram showing the number of colonies exhibiting different ranges of isobutanol production, out of the same 24 randomly selected strains from the unsorted (blue), first round sorted (red), or second round sorted (green) populations shown in (a). Source data are provided as a Source Data file.

Back to article page