Fig. 5: MiR-365 shapes the human cardiac AP by repressing the AP-regulatory network.

a RNA-seq experimental scheme and filtering steps for shortlisting the expressed genes and the differentially expressed genes for downstream gene set enrichment analysis (2) and GO term identification (1), respectively. b Functionally grouped gene ontology network of biological processes significantly associated with miR-365 target genes. Each bubble (network node) represents a specific biological process or pathway, and lines (network edges) show the relations between different terms. Nodes of the same color belong to functionally similar terms or pathways. The node size represents the term enrichment significance (P-value < 0.0005 to <0.05). c, d Testing of regulation of the GO terms voltage-gated potassium channel activity (c) and regulation of cardiac muscle cell action potential (d) by gene set enrichment analysis (GSEA) upon comparing the transcriptomes of hiPSC-CMs treated with mimic-365 or mimic-Ctrl Genes comprising each GO term are shown in the heatmaps were a spectrum of blue to red depicts low to high expression of each gene. Normalized enrichment score (NES) determines whether the term is negatively or positively enriched upon miR-365 elevation. A false discovery rate (FDR) of less than 0.05 is considered as significant enrichment.