Fig. 6: H3b ADP-ribosylation is required for faithful completion of cytokinesis.
From: Linking DNA repair and cell cycle progression through serine ADP-ribosylation of histones
a Ax2, h3b− cells, or h3b− cells expressing Flag-H3bWT or Flag-H3bS10T S28A expressing GFP-H2B were assessed for anaphase bridges during cytokinesis in either untreated cells, or cells exposed to phleomycin. A representative image of an anaphase bridge is illustrated (left panel) and quantification of data from three independent experiments (right panel). Scale bars: 10 μm. b Ax2, h3b− cells, or h3b− cells expressing Flag-H3bWT or Flag-H3bS10T S28A expressing GFP-H2B were assessed for the presence of micronuclei in either untreated cells, or cells exposed to phleomycin. A representative image of a micronucleus is illustrated (left panel) and quantification of data from 3 independent experiments (right panel). Scale bars: 5 μm. Source data are provided in the Source Data file.
