Table 1 TAP-MS data for AtEPL1B combined with metaanalysis of the previously published AP-MS experiments using NuA4 subunits as baits.

From: NuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis

 

Sc name

At gene

At name

AtARP4

AtARP4

AtSWC4

HAM1

HAM2

AtEPL1A

AtEPL1B

AtYAF9A

AtEPL1B

Complexes

NuA4, SWR1, SWI/SNF, INO80

NuA4, SWR1, SWI/SNF, INO80

NuA4, SWR1

NuA4, PEAT

NuA4, PEAT

NuA4

NuA4

NuA4, SWR1

NuA4

Model

Cell culture

Cell culture

Cell culture

Seedlings

Seedlings

Seedlings

Seedlings

 

Seedlings

Promoter

CaMV35S

UBQ10

UBQ10

Native

Native

Native

Native

CaMV35S

Native

Tag

GS TAP tag

Strep

Strep

Myc

Myc

Flag

Flag

GH TAP tag

GS TAP tag

Reference

Vercruyssen

Bieluszewski

Bieluszewski

Tan

Tan

Tan

Tan

Crevillen

This work

Conserved NuA4 subunits

ARP4

AT1G18450

AtARP4

+

+

+

+

+

+

+

+

2/1.78

 

AT1G73910

AtARP4A

+

+

SWC4

AT2G47210

AtSWC4

+

+

+

+

+

+

2/1.63

YAF9

AT5G45600

AtYAF9A

+

+

+

+

+

+

+

+

2/1.42

 

AT2G18000

AtYAF9B

+

+

EAF1

AT3G24880

AtEAF1A

+

+

+

+

+

+

+

2/0.42

 

AT3G24870

AtEAF1B

+

+

+

2/0.43

TRA1

AT2G17930

AtTRA1A

+

+

2/0.37

 

AT4G36080

AtTRA1B

+

+

+

+

2/0.44

ESA1

AT5G64610

HAM1

+

+

+

+

+

+

2/0.95

 

AT5G09740

HAM2

+

+

1/0.16

EPL1

AT1G16690

AtEPL1A

+

+

+

 

AT1G79020

AtEPL1B

+

+

+

+

2/2.22

YNG2

AT1G54390

AtING2

+

+

+

+

+

2/1.50

EAF6

AT4G14385

AtEAF6

+

+

+

+

+

2/1.96

EAF3

AT4G37280

MRG1

+

+

2/0.29

 

AT1G02740

MRG2

EAF5

EAF7

AT1G26470

AtEAF7

+

Additional AtEPL1B interactors detected here

 

AT2G31810

AHASS1

2/0.21

 

AT3G07810

AOG2

+

2/0.18

 

AT3G60110

BRD6

+

+

2/0.06

 

AT1G48520

GATB

1/0.06

 

AT1G26570

UGD1

1/0.04

Number of additional bait-specific hits

2

454

1104

331

280

96

241

114

0

  1. NSAF normalized spectral abundance factor.
  2. Numbers in the rightmost column indicate the number of biological replicates (out of two) in which the protein was detected as well as its relative abundance in the final eluate analyzed by mass spectrometry.
  3. Values in bold indicate proteins for which peptides were detected in both biological replicates.