Fig. 2: The contribution of non-ribosomal peptides and polyketides to within genus competition. | Nature Communications

Fig. 2: The contribution of non-ribosomal peptides and polyketides to within genus competition.

From: Resolving the conflict between antibiotic production and rapid growth by recognition of peptidoglycan of susceptible competitors

Fig. 2

The toxicity of WT B. subtilis strain harboring NRP single mutants ∆srfAA (surfactin), ∆pks (bacillaene), ∆bac (bacilysin), ∆pps (plipastatin), a double mutant for ∆srfAA and ∆pks, and a quadruple mutant for all NRPs/PKS operons ∆srfAA, ∆pks, ∆bac and ∆pps (∆4) towards indicated Bacilli was evaluated. Biofilm cells were harvested at 48 h post inoculation and colony-forming units (CFU) were calculated alone, and during co-inoculation. Arbitrary Units (arb. units) for toxicity were determined as the ratio of Bacilli CFU in isolation /CFU in competition against WT B. subtilis: Graphs represent mean ± SD from three independent experiments (n = 9). Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparison post hoc testing. P < 0.05 was considered statistically significant. Significant differences between the toxicity towards WT B. subtilis and toxicity towards indicated Bacilli, when competed against the indicated NRPs/PKS mutants are shown by their respective p values. Dashed Line: The toxicity exhibited by WT B. subtilis. Source data are provided as a Source Data file.

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