Fig. 2: Dynamic polar distribution of KOIN and IRK upon GT cell divisions. | Nature Communications

Fig. 2: Dynamic polar distribution of KOIN and IRK upon GT cell divisions.

From: Distinct mechanisms orchestrate the contra-polarity of IRK and KOIN, two LRR-receptor-kinases controlling root cell division

Fig. 2

a Schematics of a portion of a WT root tip highlighting formative and proliferative cell divisions during GT development. (Left to right) Formative transverse anticlinal division of the CEI (green) followed by periclinal formative division of the CEID (pink) to form endodermal and cortex cells. GT cells then proliferate through transverse anticlinal cell divisions (green). b, d, f, h, j–o) Adjacent panels show KOIN-mCherry (mCh, magenta) and IRK-GFP (green) driven by SCRp with fluorescence images merged and then each channel alone. b, d, f, h KOIN-mCh and IRK-GFP localization during proliferative transverse anticlinal endodermal cell divisions. c, e, g, i Graphs of fluorescence intensity gray values (arbitrary units) across the new PM represented in µm (dashed arrow as shown in (b): IRK-GFP (green lines) and KOIN-mCh (magenta lines). j–o KOIN-mCh and IRK-GFP localization in the CEI (j) before and (k–l) during formative transverse anticlinal cell division and in the CEID (m–o) during formative periclinal cell division. Note the CEI, cortex, and endodermal cells have elongated in (o). White arrows indicate fluorescence detected at new PMs. Scale bars: 10 µm. Images are representative of studies that were performed at least three times with at least 15 plants per condition per replicate.

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