Fig. 4: KOIN and IRK sorting determinants reside in different portions of the protein.

a Diagrams of IRK (green) and KOIN (purple) proteins with their extracellular signal peptide (SP) and LRR domains, transmembrane (TM) domain, and kinase domain (KD), and the KOIN-IRK protein chimeras. b Confocal micrographs showing WT root tips expressing SCRp driven KOIN-IRK chimeras with adjacent panels showing GFP + PI merged or GFP alone (GFP intensity color scale, PI gray). Arrowheads indicate the non-polar distribution of this chimera. c Diagrams of KOIN full-length and C-terminal truncation missing the juxtamembrane (Jx) domain and Jx and KD (JxKD). d Confocal images WT root tips expressing SCRp-driven KOIN-mCh (left) and KOINΔJxKD-mSCarlet (mSC, right) with FP shown in intensity color scale. Arrowheads indicate protein distribution. e Diagrams of IRK (green) and KOIN (purple) protein domains and the IRK-KOIN chimeras. f Confocal images of WT root tips expressing SCRp driven IRK-KOIN chimeras and stained with PI (gray) (merged, left) and GFP fluorescence alone (intensity color scale, right). g Diagrams of IRK full-length and C-terminal truncations missing the KD or the Jx and KD (JxKD). h Confocal images of WT root tips expressing SCRp driven IRK truncations with adjacent panels showing GFP + PI merged (left) or GFP alone (right). The images are representative of the observations from experiments performed at least three times with at least 15 plants per genotype and at least 3 independent transgenic lines were analyzed. Scale bars: 10 µm. Abbreviations: (Ep) epidermis; c cortex; e endodermis, (CEI) cortex/endodermal initial, and (CEID) CEI daughter.