Fig. 5: IRK∆KD is polarized in and partially rescues the cell division phenotypes in irk-4. | Nature Communications

Fig. 5: IRK∆KD is polarized in and partially rescues the cell division phenotypes in irk-4.

From: Distinct mechanisms orchestrate the contra-polarity of IRK and KOIN, two LRR-receptor-kinases controlling root cell division

Fig. 5

a, b Schematics of portions of the Arabidopsis root tip in a transverse and b median longitudinal sections. c–g Confocal images of transverse (upper, with endodermal cells highlighted) and median longitudinal (lower) optical sections from root tips stained with PI (gray) and c, g GFP with intensity color scale and PI (merged) and GFP fluorescence alone. c irk-4 root expressing IRKp:IRK-GFP. d–f Roots of WT, irk-4, and irk-1. g irk-4 roots expressing IRKp:IRK∆KD-GFP. Endodermal longitudinal anticlinal (cyan) and periclinal (magenta) cell divisions are indicated. Quantification of h longitudinal anticlinal and i periclinal endodermal cell divisions and (j) stele area of WT, irk-4 and irk-1 roots and irk-4 roots expressing IRK or IRK∆KD fusions under IRKp (n = 20 roots per genotype). Graphs h–j show representative results of experiments performed in ≥3 independent replicates with similar significant results, boxes delimit upper and lower quartiles, median values are indicated with a line, and single values are shown as dots. Bars indicate max/min values and 1–4 stars statistical significance P value < 0.05 (Brown–Forsythe one-way ANOVA test). Scale bars: 20 µm.

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