Fig. 6: Endodermal expression of KOIN-IRK chimera results in additive cell division phenotypes in irk-4. | Nature Communications

Fig. 6: Endodermal expression of KOIN-IRK chimera results in additive cell division phenotypes in irk-4.

From: Distinct mechanisms orchestrate the contra-polarity of IRK and KOIN, two LRR-receptor-kinases controlling root cell division

Fig. 6

a–f Confocal images of median longitudinal and/or transverse sections of roots stained with PI (gray) and/or fluorescence (intensity color scale). a irk-4 roots showing endodermal longitudinal anticlinal (cyan arrowheads) and periclinal (magenta arrowheads) cell divisions. b irk-4 roots expressing SCRp:IRK:GFP (GFP + PI merged) and GFP alone. c, d irk-4 roots expressing SCRp:KOINLRRsIRKTM-KD-GFP at c 7 and d 4 d.p.s. showing GT disorganization. SCRp driven GFP signal is detected in cells that positionally correspond to the cortex as they are immediately adjacent to the epidermis but have undergone many longitudinal anticlinal cell divisions. White arrowheads indicate an apparent discontinuity in endodermal cell files/rings. d A root with cells immediately interior to the epidermis that express the SCRp driven chimera with cells interior to those that morphologically resemble cortex (yellow arrowheads). e, f irk-4 roots expressing SCRp:KOIN-mCh g stained with PI (gray) and showing h KOIN-mCh localization (mCh intensity false-colored). Images correspond to representative observations of experiments replicated at least 3 times using ≥15 plants/genotype. Scale bar: 20 µm.

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