Fig. 5: Summary of the engineering strategies applied in this work to the stambomycin PKS. | Nature Communications

Fig. 5: Summary of the engineering strategies applied in this work to the stambomycin PKS.

From: Engineering the stambomycin modular polyketide synthase yields 37-membered mini-stambomycins

Fig. 5: Summary of the engineering strategies applied in this work to the stambomycin PKS.The alternative text for this image may have been generated using AI.

Inset (panels a–f) are the six distinct approaches used, and the structures of the resulting metabolites are shown. The strategies giving rise to the target mini-stambomycins 12–14 are indicated in red lettering. The hydroxyl group shown in pink is introduced by the P450-hydroxylase SamR0478, and that in red, by SamR0479. As in Figs. 1 and 3, the indicated configurations have been extrapolated from those assigned to the stambomycins 177. KS ketosynthase (KSQ refers to replacement of the active site cysteine residue by glutamine), AT acyl transferase, ACP acyl carrier protein, DH dehydratase, ER enoyl reductase, KR ketoreductase, TE thioesterase, CDD C-terminal docking domain, NDD N-terminal docking domain.

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