Fig. 3: Determination of the in vivo biochemical function of PECPS by transient expression of PECPS in N. benthamiana and knockdown expression of PECPS in A. sinensis calli. | Nature Communications

Fig. 3: Determination of the in vivo biochemical function of PECPS by transient expression of PECPS in N. benthamiana and knockdown expression of PECPS in A. sinensis calli.

From: Identification of a diarylpentanoid-producing polyketide synthase revealing an unusual biosynthetic pathway of 2-(2-phenylethyl)chromones in agarwood

Fig. 3: Determination of the in vivo biochemical function of PECPS by transient expression of PECPS in N. benthamiana and knockdown expression of PECPS in A. sinensis calli.The alternative text for this image may have been generated using AI.

a MS chromatograms (EIC) of 5-hydroxy-1,7-bis(4-hydroxyphenyl)heptan-3-one (1R) and 2-(4-hydroxyphenylethyl)-4H-chromen-4-one (P1) accumulated in N. benthamiana leaves: (i) standard of P1; (ii) leaves infiltrated with benzoyl-CoA and Agrobacterium harboring pCAMBIA1300-35S-pecps; (iii) Leaves infiltrated with Agrobacterium harboring pCAMBIA1300-35S-pecps; (iv) leaves infiltrated with Agrobacterium harboring pCAMBIA1300-35S vector. b MS and MS2 spectra of 1R and P1. c MS chromatograms (EIC) of the major PECs 1–7 (structures could be found in Supplementary Fig. 22) accumulated in A. sinensis calli treated by different methods: (i) treated with 150 mM NaCl; (ii) knockdown PECPS expression and treated with 150 mM NaCl; (iii) healthy calli. d The relative contents (1,5-diphenylpentane-1,3-dione was implemented as internal standard, IS) of PECs 1–7 accumulated in different A. sinensis calli. Data represent the mean ± SD (n = 3). Statistical analysis was performed with unpaired two-tailed Student’s t-tests. Significance is shown with asterisks: ****p < 0.0001, ***p < 0.001, **p < 0.01. Exact p-values and source data are provided in the source data file.

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