Fig. 3: Scope for the integrated chemo- and biocatalytic synthesis of aromatic amides.
From: Merging enzymes with chemocatalysis for amide bond synthesis
Reaction conditions: 1. Nitrile (50 mM), E. coli (CGA009) whole cells in 0.1 M KPi buffer (pH = 7.8)/10% v/v iPrOH (4 mL) at RT, 24 h; 2. CuBr2 (10 mol%), L1 (20 mol%), d-glu (20 mol%), iodoarene (150 mM) at 50 °C, 24 h under N2 atmosphere (Method A). Yields refer to isolated products after column chromatography. [a] Amide 12 was synthesised using PhCN (150 mM) and iodoarene (50 mM). [b] Amide 24 was synthesised using E. coli (CGA009) whole cells for 48 h.
