Fig. 5: Integrated chemo- and biocatalytic synthesis of chiral amides.
From: Merging enzymes with chemocatalysis for amide bond synthesis
A Scope of the kinetic resolution of racemic nitriles (Method C). Yields refer to isolated products after column chromatography. TPGS-750-M dl-α-Tocopherol methoxypolyethylene glycol succinate; e.r. enantiomeric ratio. B Docking of nitrile 64 into the active site of AJ270 (pdb code: 2QDY) in a putative active conformation indicates a substrate-binding pose with coordination of the nitrile group to the Fe-centre, with stereoselectivity is controlled by binding of the propyl substituent to a hydrophobic pocket formed by M40, Y72, and Y76. Labelled active site residues (blue) and substrate 64 (green).
