Fig. 3: Skeletal muscle-specific STX4 enrichment in HFD-fed mice impacts metabolism and spontaneous physical activity. | Nature Communications

Fig. 3: Skeletal muscle-specific STX4 enrichment in HFD-fed mice impacts metabolism and spontaneous physical activity.

From: Enrichment of the exocytosis protein STX4 in skeletal muscle remediates peripheral insulin resistance and alters mitochondrial dynamics via Drp1

Fig. 3

Metabolic caging analyses of the male mice used in the stages of the feeding paradigm of Fig. 2a; caging data were collected over 24–48 h. a Respiratory exchange ratio (RER) time-course over 24 h, with corresponding area under the curve (AUC) calculated as the area under each curve and 0.7 set as the baseline. ****p < 0.0001; ++++p < 0.0001; **p = 0.0077 by one-way ANOVA with Tukey’s post hoc test. b Energy expenditure time course over 24 h, with corresponding area under the curve calculation. **p = 0.0080 by one-way ANOVA with Tukey’s post hoc test. c Average hourly spontaneous activity measured as Distance K during the day (6:00–18:00) and night (18:00–6:00). All data in panels a-c are representative of Chow=8, HFD = 16, HFD + STX4 = 6. *p = 0.0120; **p = 0.0083 by one-way ANOVA with Tukey’s post hoc test. d) Extracellular flux analysis (Seahorse) to assess the mitochondrial oxygen consumption rate of HFD (n = 3, orange) and HFD + STX4 (n = 3, green) mice. Inset panel: plot of the change in maximal respiration between the HFD and HFD + STX4 groups. Box plots indicate median (middle line), 25th, 75th percentile (box) and minimum and maximum (whiskers). *p = 0.0158, 0.0493, 0.0217, 0.0291, 0.0256 from left to right, ***p = 0.0001 by unpaired two-tailed t-test. Error bars in (a–d) represent mean ± SEM. Source data are provided as a Source Data file.

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