Fig. 8: Macrophage depletion reduces dormancy in primary tumors, CTCs, and disseminating tumor cells in vivo.
a Representative immunofluorescence images of E0771-GFP primary tumor tissues treated with either control liposomes or clodronate liposomes and stained for macrophages: IBA-1 = White; DAPI = Blue. Scale bar for Primary Tumor = 100 μm. Mϕ = Macrophage. b Percentage of IBA-1 positive macrophages in 10 fields of view (1088 × 629 μm2) in each group from Fig. 8a. Control Liposomes: n = 60 HPFs in 6 animals. Clodronate liposomes: n = 50 HPFs in 5 animals. Bar = mean. Error bars = ±SEM. Unpaired t-test (p = 0.0087). **p < 0.01. c Representative immunofluorescence images of NR2F1 expression in primary tumors, circulating tumor cells (CTCs), and disseminated tumor cells (Lung) from an E0771-GFP SM model treated with control (Left) or clodronate liposomes (Right). Green = GFP, Red = NR2F1, Blue = DAPI. Scale bar for Primary Tumor = 50 μm. Scale bar for CTCs and Lung = 15 μm. d Percentage of NR2F1-positive tumor cells in each group from Fig. 8c. Control Liposomes - Primary Tumor: 3048 cells in 119 fields of view (65 × 65 µm2) in 8 animals; CTCs: n = 139 cells in 5 animals; Lung: n = 166 cells in 7 animals. Clodronate Liposomes - Primary Tumor: n = 2,298 cells in 79 fields of view (65 × 65 µm2) in 6 animals, CTCs: n = 293 cells in 6 animals; Lung: n = 190 cells in 7 animals. For primary tumor (p = 0.007), a Mann-Whitney test was used. For CTCs (p = 0.038) and Lungs (p = 0.0029), unpaired t-tests were used. *p < 0.05. **p < 0.01. Source data are provided as a Source Data file.
