Fig. 6: MuvB associates with nucleosomes in DREAM-regulated gene promoters in arrested HCT116 cells.

a Diagram of the MNase-ChIP experiment designed to enrich nucleosome-sized DNA fragments that interact with MuvB complexes. b Gene ontology (GO) analysis of the enriched DNA sequences following MNase digestion and precipitation from arrested cell extracts. Gene groups with p-values < 1 × 10⁻³⁰ are labeled. A complete list of GO terms is provided as Source data. c Top enriched genes with DNA sequences that co-precipitated with Strep-LIN9. Experiments were performed using WT or LIN37^−/− HCT116 cells, WT LIN9 or LIN9^4x, and in arrested or cycling cells. The number of genes with an enrichment >4.7-fold are indicated for each experiment. The list of enriched genes can be found on the NCBI GEO database (accession GSE189435). The annotated list of DREAM genes used as a cross-reference is provided as Source data. d Genome browser tracks corresponding to the CCNB2, FOXM1, and ORC6 promoters. The number of DNA sequence reads is plotted for the input (gray) and Strep-LIN9 precipitated DNA samples. These data correspond to one replicate performed in arrested HCT116 cells. Data for other replicates and experiments are shown in Supplementary Fig. 6. The transcription start site (TSS) in each gene (base of orange arrow) along with the position of the DREAM-binding DNA motif relative to the TSS are indicated.