Fig. 7: The sharp positioning of the MuvB-bound +1 nucleosome correlates with gene repression. | Nature Communications

Fig. 7: The sharp positioning of the MuvB-bound +1 nucleosome correlates with gene repression.

From: The MuvB complex binds and stabilizes nucleosomes downstream of the transcription start site of cell-cycle dependent genes

Fig. 7

a Heatmaps of relative read density across all known DREAM genes that show >4.7-fold enrichment in the Strep-LIN9 precipitant and correspond. Both input and precipitant sequencing data are shown for the same gene set and in the same order. At the bottom are the aggregated and normalized read intensities across all genes shown in the heat map for the input and precipitated DNA data sets. Data from HCT116 (blue) and HCT116-LIN37−/− (pink) are shown. The observation of more peaks with periodicity corresponding to nucleosomes (i.e., the +2 and +3 nucleosome peaks) in the HCT116-LIN37−/− input data set is consistent with DREAM genes being active during Nutlin-3a-induced quiescence of those cells45. b Comparison of three example gene tracks showing the number of DNA sequence reads in Strep-LIN9 precipitated samples from HCT116 (blue, left axis) and HCT116-LIN37−/− (pink, right axis) cells. The TSS (orange arrow) and DREAM-binding DNA motifs are indicated. c Overall model showing organization of DREAM and the association between MuvBN and the +1 nucleosome, which we propose mediates gene repression.

Back to article page