Fig. 6: Summary of sequencing results from non-functional kill switches and potential mechanisms of circuit inactivation.
From: Genetically stable CRISPR-based kill switches for engineered microbes
a Potential mechanisms of kill switch inactivation and cell survival. DSB, double-strand break. b Classifications of the observed mutations in Ptet-cas9, Ptet-gRNA, tetR, and tlpA expression cassettes. Source data are provided as a Source Data file. c Sequence of the most commonly observed inactivation mutation in the utilized Ptet promoters.
