Fig. 1: vFBs are activated after long but not short courtship experience.
From: A neural circuit linking learning and sleep in Drosophila long-term memory
a Mean normalized luminescence traces (±SEM) in vFBs. b Mean normalized luminescence in the 1–3 h time period after training (TP1-3) in a. n0 h = 33, n1 h = 29, n2 h = 37, n4 h = 35, n6 h = 35, P1 h = 0.7824, P2 h = 0.2996, P4 h = 2.9e−5, P6 h = 0.0065 for H0 N. Lum.exp = N. Lum.nai, P = 0.0014 for H0 N. Lum.2 h = N. Lum.4hr, P = 0.6311, for H0 N. Lum.4 h = N. Lum.6 h, two-sided Student T test. c Mean sleep traces of ΔSleep (exp-nai) (±SEM) after training. d Mean ΔSleep (exp-nai) in TP1-3 in c. n0 h = 45, n1 h = 42, n2 h = 41, n4 h = 44, n6 h = 48, P1 h = 0.5480, P2 h = 0.6338, P4 h = 0.0022, P6 h = 1.1e−4 for H0 ΔSleep = 0, two-sided Wilcoxon Signed Rank test, and P = 0.0251 for H0 ΔSleep2 h = ΔSleep4 h, P = 0.4809 for H0 ΔSleep4 h = ΔSleep6 h, two-sided Wilcoxon Rank Sum test. e LTM, shown as Suppression Index, SI [%] of males trained as indicated in a. n0 h = 58, n1 h = 60, n2 h = 59, n4 h = 63, n6 h = 60, P1 h = 0.2942, P2 h = 0.3277, P4 h = 0.0022, P6 h = 1.2e−4 for H0 SI = 0, and P = 0.0175 for H0 SI2 h = SI4 h, two-sided Permutation test. f (top) Mean sleep traces of ΔSleep (exp-nai) (±SEM) upon vFBs silencing. (bottom) Mean ΔSleep (exp-nai) in TP1-3 of the data at the top. (left) n20 °C = 57, n30 °C = 59, P20 °C = 1.4e−4, P30 °C = 0.0581 value is for H0 ΔSleep = 0, and P = 2.6e−5 for H0 ΔSleep20 °C = ΔSleep30 °C, (middle) n20 °C = 63, n30 °C = 46, P20 °C = 1.6e−6, P30 °C = 1.4e−6 for H0 ΔSleep = 0, and P = 0.1399 for H0 ΔSleep20 °C = ΔSleep30 °C, (right) n20 °C = 59, n30 °C = 65, P20 °C = 0.0019, P30 °C = 1.3e−9 for H0 ΔSleep = 0, and P = 0.0612 for H0 ΔSleep20 °C = ΔSleep30 °C. Two-sided Wilcoxon Signed Rank test for single groups and two-sided Wilcoxon Rank Sum test across groups. Full genotypes and data analysis details can be found in Supplementary Tables 1–3. Source data are provided as a Source Data file. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001. n represents independent fly samples with assays repeated at least 3 times (b, d, e and f). Box plots represent median and IQR and whiskers extend to lower and upper adjacent values (b, d and f).
